Survival of immunised mice after a lethal tachyzoite challenge. Groups of mice were intraperitoneally immunised with the recombinant antigens in combination with the Freund’s adjuvant (light blue), mindin adjuvant (green), PBS (yellow), or Freund’s adjuvant alone (pink), mindin adjuvant alone (black), and PBS alone (grey). Nine weeks after the immunisation, all experimental mice were intraperitoneally infected with 250 live tachyzoites of T. gondii (RH strain). Animals were observed daily for 20 days, and the final survival rates were recorded. (A): Mouse group immunised with rBAG1 antigen and controls; (B): Mouse group immunised with rSRS4 antigen and controls; (C): Mouse group immunised with rSRS9 antigen and controls. Mice immunised with the recombinant proteins in combination with either Freund’s adjuvant or mindin showed most prolonged survival time. The Kaplan-Meier survival curves were generated with the MedCalc statistical software (http://www.medcalc.org/manual/kaplan-meier.php).