A multiplex nested PCR for the detection and identification of Candida species in blood samples of critically ill paediatric patients

Table 1 Primers employed in the nested multiplex PCR amplifications

Primers	Sequences (5’ → 3’)	Amplicons	References
ITS 1/4	F- TCCGTAGGTGAACCTGCGG	Variable	[18]
	R- TCCTCCGCTTATTGATATGC
CALB 1/2	F- TTTATCAACTTGTCACACCAGA	272 bp	[18]
	R- ATCCCGCCTTACCACTACCG
CGL 1/2	F- TTATCACACGACTCGACACT	423 bp	[18]
	R- CCCACATACTGATATGGCCTACAA
CTR 1/2	F- CAATCCTACCGCCAGAGGTTAT	357 bp	[18]
	R- TGGCCACTAGCAAAATAAGCGT
CPAR 3/2	F- GCCAGAGATTAAACTCAACCAA	297 bp	[18]
	R- CCTATCCATTAGTTTATACTCCGC
CKR 2/3	F- ACTACACTGCGTGAGCGGAA	362 bp	[19]
	R- ACTACACTGCGTGAGCGGAA
CLU 1/2	F- GCGATACGTAGTATGACTTGCAG	137 bp	*
	R- GATATTTCGGAGCAACGCCTAACC
CPEL 1/2	F-GAACTTTGCTTTGGGTGGTGAG	160 bp	*
	R- CTTCATCGTTGCGAGAACCAAG

F = forward; R = reverse; CALB = C. albicans; CGL = C. glabrata; CTR = C. tropicalis; CPAR = C. parapsilosis complex; CKR = C. krusei; CLU = C. lusitaniae; CPEL = C. pelliculosa.
* = primers designed in the current study; bp = base pairs.

ISSN: 1471-2334