Figure 1

Agarose gel showing the nested multiplex PCR products obtained from patients after amplification with primers for assays 1 and 2. Lane L – 100 bp molecular weight marker (GE Healthcare); Lanes 1 and 8 – Negative controls; Lanes 2 and 9 – Patient 2 (C. albicans); Lanes 3 and 10 – Patient 8 (C. albicans); Lanes 4 and 11 – Patient 11 (C. tropicalis); Lanes 5 and 12 – Patient 13 (C. tropicalis and C. parapsilosis); Lanes 6 and 13 – Patient 7 (C. krusei); Lane 7 – Positive controls for assay 1: DNA from C. glabrata (423 bp), C. tropicalis (357 bp), C. albicans (272 bp) and C. lusitaniae (137 bp) reference strains; Lane 14 – Positive controls for assay 2: DNA from C. krusei (362 bp), C. parapsilosis (297 bp) and C. pelliculosa (160 bp) reference strains.