Table 2 Deep sequencing of cloned HVR1 sample

Number of reads of cloned plasmid (control)

3178

Number of variants

12

Most abundant erroneous variant

1.48%

Least abundant erroneous variant

0.06%

Overall error rate per base

0.05%

Types of errors:

Insertions

0.04%

Substitutions

0.006%

Deletions

0.002%

Overall insertions at homopolymeric regions

51%

Number of variants after ShoRAH

4

Most abundant erroneous variant after ShoRAH

0.5%

Least abundant erroneous variant after ShoRAH

0.2%