Figure 1From: Humoral immune response to Shiga Toxin 2 (Stx2) among Brazilian urban children with hemolytic uremic syndrome and healthy controls Reactivity profile against Stx2 by Immunoblotting (WB) in HUS patients and healthy children (controls). A) Each bar represents samples reactive to A and B subunits or only A subunit or non-reactive. * Significant differences were observed between the groups (p = 0.032). B) Representative immunoblotting strips from HUS patients (lanes 3 and 4) and control sera (lanes 5 and 6) reactive to Stx2. Immunoblotting reaction was carried out by incubating nitrocellulose membranes, containing purified Stx2 toxin subjected to 12.5% SDS-PAGE, with sera from HUS patients and healthy individuals followed by incubation with goat anti-human IgG. Lanes 1 and 2 represent positive and negative controls of the assay, respectively, purified toxin incubated with rabbit anti-Stx2 polyclonal antibody (lane 1) or pre-immune rabbit serum (lane 2), followed by peroxidase-conjugate anti-rabbit IgG. The arrows represent the A subunit (32 kDa) and B subunit (7.5 kDa) of Stx2.Back to article page