Figure 2
From: Serum biomarkers predictive of cure in Chagas disease patients after nifurtimox treatment
Immunoblot analysis of sera using rabbit anti-neoepitope antisera. Immunoblot analysis of sera from healthy controls (HC), 2008 chagasic samples (2008-CD+) and 2011 follow-up samples (2011-FU) probed with specific rabbit neo-epitope antisera. (A) Analysis of pooled sera and (B) individual sera with anti-APOA1(24) demonstrates the upregulation of the 24.7 kDa APOA1 fragment in 2008-CD + samples and its down-regulation back to healthy controls level in the 2011-FU samples. The specificity of the rabbit anti-APOA1(24) serum for the 24.7 kDa fragment is demonstrated by its inability to detect native full-length APOA1 (~28.1 kDa) (FL) while reacting with a 6xHis recombinant of the 24.7 APOA1 fragment (~27.4 kDa) (R). (C) Analysis of pooled and (D) individual sera with rabbit anti-FN1(28) serum demonstrates the up-regulation of the 28.9 kDa FN1 fragment in 2008-CD + samples and its down-regulation in the 2011-FU samples almost back to the levels observed in healthy controls. The specificity of the rabbit anti-FN1(28) serum for the 28.9 kDa fragment is demonstrated by its inability to detect native full-length FN1 (~210 and ~220 kDa) (FL) while reacting with a FLAG-6xHis recombinant of the 28.9 FN1 fragment (~35 kDa) (R).