Cluster plots of selected differentially expressed biomarkers. Cluster plots demonstrate the expression pattern of selected biomarkers between the different groups (HC = healthy controls; 2008-CD + = chagasic samples; 2011-FU = follow-up samples from patients having received treatment with Nfx). Biomarker expression levels in samples treated with Nfx (2011-FU) all return to the levels observed in healthy controls (HC). All biomarkers, except for one, are upregulated during disease (2008-CD+). The downregulated biomarker was previously identified as native human APOA1 (28.1 kDa); the 9.3, 13.6 and 24.7 kDa biomarkers were identified as fragments of human APOA1; and the 28.9 kDa biomarker as a fragment of human FN1.