Detection of a divergent Parainfluenza 4 virus in an adult patient with influenza like illness using next-generation sequencing

Table 1 PIV4 PCR oligomer mismatches to isolate QLD-01

Primer name	Sequence (5′-3′)	Study
PI4P+	CTGAACGGTTGCATTCAGGT	[3]
PIASB+	AACCAGGGAAACAGAGCTC	[3]
LPW 1779	GTGTCTGATCCCATA AGCAGC	[5]
PIV-4 Reverse	GCATGTTCTGCATCTCTGGA	[16]
PIV 4 Forward	CAAATGATCCACAGCAAAGATTC	[18]
PIV 4 Reverse	ATGTGGCCTGTAAGGAAAGCA	[18]
PIV 4 Probe	GTATCATCATCTGCCAAATCGGCAATTAAACA	[18]

Published PIV4 primer and probe sequences with mismatches to isolate QLD-01 underlined and in bold.

ISSN: 1471-2334