Table 1 Primers used for PCR to detect Shigella resistance and virulence genes
Primer | Sequence (5′-3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
ESBL genes | |||
CTX-M-1 group | F-GGCCCATGGTTAAAAAATCACTGC | 891 | [3] |
| Â | R-CCGTTTCCGCTATTACAAACCGTTG | Â | |
CTX-M-2 group | F-CTCAGAGCATTCGCCGCTCA | 843 | |
| Â | R-CCGCCGCAGCCAGAATATCC | Â | |
CTX-M-8 group | F-ATGATGAGACATCGCGTTAAC | 864 | |
| Â | R-CGGTGACGATTTTCGCGGCAG | Â | |
CTX-M-9 group | F-GTGACAAAGAGAGTGCAACGG | 856 | |
| Â | R-ATGATTCTCGCCGCTGAAGCC | Â | |
OXA group | F-ACACAATACATATCAACTTCGC | 813 | |
| Â | R-AGTGTGTTTAGAATGGTGATC | Â | |
SHV group | F-GCCTTTTCGGCCTTCACTCAAG | 989 | |
| Â | R-TTAGCGTTGCCAGTGCTCGATCA | Â | |
TEM group | F-ATAAAATTCTTGAAGACGAAA | 1076 | |
| Â | R-GACAGTTACCAATGCTTAATC | Â | |
AmpC β-lactamase genes | |||
MOX group | F-GCTGCTCAAGGAGCACAGGAT | 520 | [14] |
| Â | R-CACATTGACATAGGTGTGGTGC | Â | |
CIT group | F-TGGCCAGAACTGACAGGCAAA | 462 | |
| Â | R-TTTCTCCTGAACGTGGCTGGC | Â | |
DHA group | F-AACTTTCACAGGTGTGCTGGGT | 405 | |
| Â | R-CCGTACGCATACTGGCTTTGC | Â | |
ACC group | F- AACAGCCTCAGCAGCCGGTTA | 346 | |
| Â | R- TTCGCCGCA ATCATCCCTAGC | Â | |
EBC group | F- TCGGTAAAGCCGATGTTGCGG | 302 | |
| Â | R- CTTCCACTGCGGCTGCCAGTT | Â | |
FOX group | F- AACATGGGGTATCAGGGAGATG | 190 | |
| Â | R- CAA AGCGCGTAACCGGATTGG | Â | |
Virulence gene | |||
ial | F-CTGGATGGTATGGTGAGG | 320 | [12] |
| Â | R-GGAGGCCAACAATTATTTCC | Â | |
ipaH | F-TGGAAAAACTCAGTGCCTCT | 423 | |
| Â | R-CCAGTCCGTAAATTCATTCT | Â | |
setlA | F-TCACGCTACCATCAAAGA | 309 | |
| Â | R-TATCCCCCTTTGGTGGTA | Â | |
setlB | F-GTGAACCTGCTGCCGATATC | 147 | |
| Â | R-ATTTGTGGATAAAAATGACG | Â | |
sen | F-ATGTGCCTGCTATTATTTAT | 799 | |
| Â | R-CATAATAATAAGCGGTCAGC | Â | |
virA | F-CTGCATTCTGGCAATCTCTTCACATC | 215 | [13] |
| Â | R-TGATGAGCTAACTTCGTAAGCCCTCC | Â | Â |