Figure 1

Differentiation of spores into spherules. (a, b) Differentiating spherical spores; (c, d) young spherules without endospores; (e) mature spherule with endospores; (f, g, h) expulsion of endospores, and (i) endospores free. (a, b) bronchial lavage and (c, d, e, f, g, h, i) sputum. (a) 40X, calcofluor white staining, and (b) 40X; (c, d, e, f, g, h, i) 100X, fresh examination with 15% KOH.