Figure 2

Tissue mycolactone concentration in serial biopsies during and after antibiotic treatment. Antibiotic treatment was started at week 0 and completed at week 8. Biopsies were taken at weeks 0 in all patients and at week 6 and/or 12 when it was clinically indicated. (A) Mycolactone concentration in ASL measured by cytotoxicity assay using synthetic mycolactone A/B generated calibration curves. (B) Tissue mycolactone concentration in serial biopsies during and after antibiotic treatment. Mycolactone A/B concentration in ASL measured by multiple reaction monitoring (MRM) methodology with liquid chromatographic separation coupled to a tandem mass spectrometer.