Table 2 Optimum conditions for detection mutation of rpoB , katG, gyrA, rrs, embB and rpsl genes by pyrosequencing
Loci | Detection mode | Nucleotide dispensation order |
|---|---|---|
rpoB | SQA mode | RRDR mutation: 16(AGCT) |
katG | SNP mode | 315 mutation: GTCACAGTCTG |
rpsl | SNP mode | 43 mutation: CGATACGAGT 88 mutation: CGATGCACGC |
embB | SNP mode | 306 mutation:TGCAGTGACGCGCAGT |
gyrA | SQA mode | QRDR mutation: 12(GACT) |
rrs | SNP mode | 1401 mutation: TCGTCAGTCAGTCAT |