Inhibition of MTB multiplication by subcutaneous vaccination with BCG-dHCM. Five-week-old C57BL/6 mice (5 mice/group) were subcutaneously vaccinated with 1 × 103 CFU/mouse of either BCG-261H or BCG-dHCM and were challenged with 100 CFU/lungs of H37Rv by aerosol infection, at 6 weeks post vaccination. The number of MTB recovered from the lung (a) or spleen (b) at 6 weeks post challenge was enumerated by colony assay method. Representative results of three separate experiments are shown. Titers were statistically compared using Student’s t-test.