Activation of APCs by BCG-dHCM. a. Cytokine production from DC by stimulation with BCG-dHCM. DC produced using rGM-CSF and rIL-4 were stimulated with either BCG-261H or BCG-dHCM for 24 h. b. Up-regulation of APC-associated molecules and activation marker on DC by infection with BCG-dHCM. Monocyte-derived immature DC were infected with either BCG-261H or BCG-dHCM at an MOI 0.25 and cultured for another two days in the presence of rGM-CSF and rIL-4. The DC from day 6 of culture were gated and analyzed. Dotted lines, isotype-matched control IgG; solid lines, the indicated test mAb. The number at the top right corner of each panel represents the difference in the fluorescence intensity between the control IgG and the test mAb. c. Cytokine production from macrophages by stimulation with BCG-dHCM. Macrophages differentiated from monocytes by using M-CSF were stimulated with either BCG-261H or BCG-dHCM for 24 h. The concentration of the indicated cytokine was determined by the ELISA method. A representative of three separate experiments conducted by using 3 different PBMCs-donors is shown. Assays were performed in triplicate and the results are expressed as the mean ± SD. Titers were statistically compared using Student’s t-test.