Glutathione S-transferase L1 multiplex serology as a measure of cumulative infection with human papillomavirus

BMC Infectious Diseases

Table 3 Sampling-adjusted agreement between GST-L1 and VLP-ELISA at GST-L1 laboratory suggested (400 MFI) and alternate cutoffs

HPV type	GST-L1 cutoff (MFI)		GST-L1 + (N)	GST-L1 – (N)	Percent agreement	Positive agreement	Kappa
HPV 16	400	VLP-ELISA +	133	407	85.1%	39.2%	0.34
	400	VLP-ELISA –	7	2,239	85.1%	39.2%	0.34
	72	VLP-ELISA +	344	197	88.7%	68.6%	0.62
	72	VLP-ELISA –	118	2,128		68.6%	0.62
	400	cLIA +	64	98	93.7%	42.1%	0.39
	400	cLIA –	76	2,547		42.1%	0.39
	72	cLIA +	122	40	86.4%	39.1%	0.33
	72	cLIA –	339	2,284		39.1%	0.33
	400	SEAP-NA +	92	296	86.8%	33.1%	0.27
	400	SEAP-NA –	77	2,349		33.1%	0.27
	72	SEAP-NA +	199	189	79.9%	41.3%	0.30
	72	SEAP-NA –	377	2,049		41.3%	0.30
HPV 18	400	VLP-ELISA +	90	618	77.1%	20.8%	0.13
	400	VLP-ELISA –	65	2,206		20.8%	0.13
	65	VLP-ELISA +	403	305	83.1%	61.5%	0.51
	65	VLP-ELISA –	199	2,072		61.5%	0.51
	400	cLIA +	43	67	94.0%	32.1%	0.29
	400	cLIA –	113	2,757		32.1%	0.29
	65	cLIA +	100	9	82.8%	28.2%	0.23
	65	cLIA –	502	2,367	82.8%	28.2%	0.23

Analyses are based on 467 women for HPV16 and 477 women for HPV18; displayed sample sizes are sampling-adjusted. Alternate GST-L1 cutoffs were calculated to maximize agreement with VLP-ELISA (see Statistical Methods).

ISSN: 1471-2334