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Table 2 Panel of organisms used for coverage and specificity analysis

From: Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

Species Strain Strain detailsa Targetsb
    BuMC psu mau cry1
Burkholderia NCTC 10229 Bird, 1961 14,5 - 19,2 -
mallei NCTC 10230 Horse, 1961 14,6 - 19,3 -
  NCTC 10245 Horse, 1972 14,1 - 18,8 -
  NCTC 10247 Turkey, 1960 15,2 - 19,8 -
  NCTC 10248 Clinical isolate, Turkey, 1950 12,2 - 17,4 -
  NCTC 10260 Clinical isolate, Turkey, 1949 16,2 - 20,8 -
  NCTC 120 1920 14,8 - 19,4 -
  NCTC 3708 Mule, India, 1932 14,8 - 19,4 -
  NCTC 3709 Horse, India, 1932 15,0 - 19,3 -
  NCTC 12938 T Clinical isolate 15,6 - 20,0 -
Burkholderia NCTC 10274 Clinical isolate, Kuala Lumpur, 1962 16,3 18,5 - -
pseudomallei NCTC 10276 Clinical isolate, 1962 16,5 19,0 - -
  NCTC 11642   14,7 18,2 - -
  NCTC 1688 Rat, Malaysia, 1923 16,9 19,2 - -
  NCTC 4845 infected laboratory monkey, Singapore,1935 - 18,8 - -
  NCTC 4846 infected laboratory monkey, Singapore,1935 15,9 18,3 - -
  NCTC 6700 Clinical isolate, 1942 15,9 18,7 - -
  NCTC 7383 1948 16,0 18,7 - -
  NCTC 7431 1948 16,0 18,3 - -
  NCTC 8016 Sheep, Queensland, 1949 17,0 18,8 - -
  NCTC 8707 Jordan, 1946 16,6 18,8 - -
  NCTC 8708 Jordan, 1946 17,8 19,9 - -
  NCTC 12939 T Clinical isolate, USA, 1953 - 18,4 - -
  BD08-00100 Clinical isolate, Netherlands, 2008c 17,0 18,3 - -
  BD08-00103 Clinical isolate, Netherlands, 2008 18,8 20,2 - -
  BD08-00268 Clinical isolate, Netherlands, 2008 16,2 19,0 - -
  BD10-00211 Clinical isolate, Netherlands, 2010 - 18,6 - -
  BD12-00016 Clinical isolate, Netherlands, 2012 18,5 21,1 - -
  BD12-00217 Clinical isolate, Netherlands, 2012 19,6 22,2 - -
Burkholderia DSM 13276 Environmental sample, Thailand - - - -
thailandensis CIP 106301 Soil, Thailand, 1994 - - - -
  CIP 106302   - - - -
Bacillus anthracis NCTC 8234 Weybridge, 1951 (Sterne) - - - -
  NCTC 10340 Cow, Edinburgh, 1963 (Vollum) - - - -
Francisella tularensis BD07-537 Clinical isolate, Netherlands, 2007 - - - -
subsp. holarctica (B)       
Yersinia pestis Kenya 164 Biovar antiqua, Kenya, <1952 - - - -
  Harbin Biovar mediaevalis, China, <1948 - - - -
  Madagascar 34-94 Biovar orientalis, Madagascar - - - -
Bacillus atrophaeus ATCC 9372   - - - -
Bacillus cereus ATCC 11778 NCIB Aberdeen, 1962 - - - -
Bacillus coagulans   Purchased at Raven Labs, USA - - - -
Bacillus megaterium ATCC 8245   - - - -
Bacillus pumilus ATCC 27142   - - - -
Bacillus subtilis ATCC 6633   - - - -
Bacillus thuringiensis ATCC 29730 var. galleriae Heimpel - - - 20,4
Enterobacter cloacae NCTC 13168   - - - -
Escherichia coli ATCC 25922 Clinical isolate, 1946 - - - -
Pseudomonas ATCC 15442   - - - -
aeruginosa ATCC 27853 Blood culture, 1969 - - - -
Klebsiella pneumoniae BD05-258 Clinical isolate, 2005 - - - -
Salmonella enterica ATCC 14028 Serovar Typhimurium. - - - -
subsp. enterica   liver, 1987 - - - -
  ATCC 13076 Serovar Enteritidis - - - -
Bos taurus 0469 Netherlands, 2009 - - - -
Chrysops relictus I Tissue, Netherlands, 2009 - - - -
Homo sapiens Volunteer 8 Blood, Netherlands, 2009 - - - -
  Volunteer 10 Blood, Netherlands, 2009 - - - -
Equus ferus caballus   Tissue, Netherlands, 2010 - - - -
Mus musculus   Netherlands, 2009 - - - -
Ovis aries Twello 67 Slaughterhouse, Netherlands, 2009 - - - -
Rattus norvegicus 08604 Netherlands, 2009 - - - -
Rattus rattus 08402 Netherlands, 2009 - - - -
Sus scrofa 566 Slaughterhouse, Netherlands, 2009 - - - -
  1. aThe names of the countries or towns are those used at the time of the strain isolation and have been kept for strain designation.
  2. bNumbers represent Cq values; - means no amplification. cAll clinical isolates from B. pseudomallei designated BD were isolated in the Netherlands from imported cases from Thailand.
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BMC Infectious Diseases

ISSN: 1471-2334

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