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Table 2 Panel of organisms used for coverage and specificity analysis

From: Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei

Species

Strain

Strain detailsa

Targetsb

   

BuMC

psu

mau

cry1

Burkholderia

NCTC 10229

Bird, 1961

14,5

-

19,2

-

mallei

NCTC 10230

Horse, 1961

14,6

-

19,3

-

 

NCTC 10245

Horse, 1972

14,1

-

18,8

-

 

NCTC 10247

Turkey, 1960

15,2

-

19,8

-

 

NCTC 10248

Clinical isolate, Turkey, 1950

12,2

-

17,4

-

 

NCTC 10260

Clinical isolate, Turkey, 1949

16,2

-

20,8

-

 

NCTC 120

1920

14,8

-

19,4

-

 

NCTC 3708

Mule, India, 1932

14,8

-

19,4

-

 

NCTC 3709

Horse, India, 1932

15,0

-

19,3

-

 

NCTC 12938 T

Clinical isolate

15,6

-

20,0

-

Burkholderia

NCTC 10274

Clinical isolate, Kuala Lumpur, 1962

16,3

18,5

-

-

pseudomallei

NCTC 10276

Clinical isolate, 1962

16,5

19,0

-

-

 

NCTC 11642

 

14,7

18,2

-

-

 

NCTC 1688

Rat, Malaysia, 1923

16,9

19,2

-

-

 

NCTC 4845

infected laboratory monkey, Singapore,1935

-

18,8

-

-

 

NCTC 4846

infected laboratory monkey, Singapore,1935

15,9

18,3

-

-

 

NCTC 6700

Clinical isolate, 1942

15,9

18,7

-

-

 

NCTC 7383

1948

16,0

18,7

-

-

 

NCTC 7431

1948

16,0

18,3

-

-

 

NCTC 8016

Sheep, Queensland, 1949

17,0

18,8

-

-

 

NCTC 8707

Jordan, 1946

16,6

18,8

-

-

 

NCTC 8708

Jordan, 1946

17,8

19,9

-

-

 

NCTC 12939 T

Clinical isolate, USA, 1953

-

18,4

-

-

 

BD08-00100

Clinical isolate, Netherlands, 2008c

17,0

18,3

-

-

 

BD08-00103

Clinical isolate, Netherlands, 2008

18,8

20,2

-

-

 

BD08-00268

Clinical isolate, Netherlands, 2008

16,2

19,0

-

-

 

BD10-00211

Clinical isolate, Netherlands, 2010

-

18,6

-

-

 

BD12-00016

Clinical isolate, Netherlands, 2012

18,5

21,1

-

-

 

BD12-00217

Clinical isolate, Netherlands, 2012

19,6

22,2

-

-

Burkholderia

DSM 13276

Environmental sample, Thailand

-

-

-

-

thailandensis

CIP 106301

Soil, Thailand, 1994

-

-

-

-

 

CIP 106302

 

-

-

-

-

Bacillus anthracis

NCTC 8234

Weybridge, 1951 (Sterne)

-

-

-

-

 

NCTC 10340

Cow, Edinburgh, 1963 (Vollum)

-

-

-

-

Francisella tularensis

BD07-537

Clinical isolate, Netherlands, 2007

-

-

-

-

subsp. holarctica (B)

      

Yersinia pestis

Kenya 164

Biovar antiqua, Kenya, <1952

-

-

-

-

 

Harbin

Biovar mediaevalis, China, <1948

-

-

-

-

 

Madagascar 34-94

Biovar orientalis, Madagascar

-

-

-

-

Bacillus atrophaeus

ATCC 9372

 

-

-

-

-

Bacillus cereus

ATCC 11778

NCIB Aberdeen, 1962

-

-

-

-

Bacillus coagulans

 

Purchased at Raven Labs, USA

-

-

-

-

Bacillus megaterium

ATCC 8245

 

-

-

-

-

Bacillus pumilus

ATCC 27142

 

-

-

-

-

Bacillus subtilis

ATCC 6633

 

-

-

-

-

Bacillus thuringiensis

ATCC 29730

var. galleriae Heimpel

-

-

-

20,4

Enterobacter cloacae

NCTC 13168

 

-

-

-

-

Escherichia coli

ATCC 25922

Clinical isolate, 1946

-

-

-

-

Pseudomonas

ATCC 15442

 

-

-

-

-

aeruginosa

ATCC 27853

Blood culture, 1969

-

-

-

-

Klebsiella pneumoniae

BD05-258

Clinical isolate, 2005

-

-

-

-

Salmonella enterica

ATCC 14028

Serovar Typhimurium.

-

-

-

-

subsp. enterica

 

liver, 1987

-

-

-

-

 

ATCC 13076

Serovar Enteritidis

-

-

-

-

Bos taurus

0469

Netherlands, 2009

-

-

-

-

Chrysops relictus

I

Tissue, Netherlands, 2009

-

-

-

-

Homo sapiens

Volunteer 8

Blood, Netherlands, 2009

-

-

-

-

 

Volunteer 10

Blood, Netherlands, 2009

-

-

-

-

Equus ferus caballus

 

Tissue, Netherlands, 2010

-

-

-

-

Mus musculus

 

Netherlands, 2009

-

-

-

-

Ovis aries

Twello 67

Slaughterhouse, Netherlands, 2009

-

-

-

-

Rattus norvegicus

08604

Netherlands, 2009

-

-

-

-

Rattus rattus

08402

Netherlands, 2009

-

-

-

-

Sus scrofa

566

Slaughterhouse, Netherlands, 2009

-

-

-

-

  1. aThe names of the countries or towns are those used at the time of the strain isolation and have been kept for strain designation.
  2. bNumbers represent Cq values; - means no amplification. cAll clinical isolates from B. pseudomallei designated BD were isolated in the Netherlands from imported cases from Thailand.
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