Table 1 Primers and probes for multiplex qPCR
Organism | Target | Oligo function | Oligo name | Sequence 5'-3' a |
|---|---|---|---|---|
B. pseudomallei + | ISBma2 | primer | Bumcpri_f | GCGGAAGCGGAAAAAGGG |
B. mallei | ISBma2transposase | primer | Bumcpri_r | GCGGGTAGTCGAAGCTG |
| Â | Â | probe | Tqpro_Bumc | FAM-TCRCCAGACGCAGCAGCAT-BHQ1 |
B. pseudomallei | Hypothetical | primer | psupri_f | GCGCGATCCGTCGAG |
| Â | protein | primer | psupri_r | AGCCGCTACGACGATTATG |
| Â | Â | probe | Tqpro_psu | JOE-CCGCGACAATACGACCATCC-BHQ1 |
B. mallei | Hypothetical | primer | maupri3_f | GGCGAAAGAACGCGAAC |
| Â | protein | primer | maupri3_r | GCGTTCCACGATCAACTCT |
| Â | Â | probe | Tqpro2_mau | CF590-CATCCCGCACCGTCCG-BHQ2 |
B. thuringiensis | Crystal protein | primer | Btpri_f | GCAACTATGAGTAGTGGGAGTAATTTAC |
| Â | gene | primer | Btpri_r | TTCATTGCCTGAATTGAAGACATGAG |
| Â | Â | probe | Tqpro_Bt | Cy5-ACGTAAATACACT-BHQ2-TGATCCATTTGAAAAG-P |