Figure 6

IgG subclass distribution against strongly recognized recombinant antigens (RI > 10). p values were calculated using Mann-Whitney's (U) test. A: IgG1 and IgG3 subclasses were predominant with differences among groups reactive to rRO33 (IgG3 asymptomatic (A) > IgG3 symptomatic (S)). The same was observed against rMSP4 with IgG1(A) > IgG1(S). IgG4 was present in reactive asymptomatic plasmas to rRO33 and in reactive symptomatic plasmas to rMSP1₁₉. B: rMSP1₁₉(IgG1(S) > IgG1(A), IgG3(S) > IgG3(A)). C: Against rMSP3-3D7-like, both groups presented IgG1 and a tendency to IgG3(A) > IgG3(S). D: A tendency to higher IgG1 in plasmas from asymptomatics was observed against rMSP4. E/F: No differences among groups to rMSP7 and rMSP10_3D7-like. IgG1, IgG3 and IgG4 were detected in symptomatic plasmas and IgG1 and IgG4 in asymptomatic plasmas with no differences among IgG subclasses for both groups. G-I: Only asymptomatic plasmas presented RI > 10 to rMSP2_IC1-69, rMSP9 and rEBA175. IgG3 were predominant in reactive asymptomatic plasmas to rMSP2_IC1-69 by Wilcoxon signed rank test and IgG1 were predominant in reactive asymptomatic plasmas to rMSP9, by Friedman’s test. No difference was observed between IgG1 and IgG3 against rEBA175 in reactive plasmas from asymptomatics.