Linear epitope mapping of FRA with human patient sera. Peptides 14 amino acid residues long with an overlap of 9 residues between peptides were tested for reactivity against a pool of sera from six Chagasic patients. (A) Image of a representative membrane revealed by chemiluminescence after immunoblotting. (B) Measured signal intensity of the spots produced from the image in Panel A. (C) Sequences of the peptides synthesized and their positions on the membrane. (D) Molecular model of the FRA protein as predicted by I-Tasser server. Visualization of proteins was generated as a “new cartoon” representation with the quality of visualization was set at the maximum value of 50. The red segments display the overlapping epitopes with highest signals amongst the rest of the repeated structure in blue.