Figure 1

Linear epitope mapping of CRA with human patient sera. Peptides consisting of 14 amino acid residues with an overlap of 9 residues between peptides were tested for reactivity against a pool of sera from six Chagasic patients. (A) Image of a representative membrane revealed by chemiluminescence after immunoblotting. (B) Measured signal intensity of the spots from membrane in Panel A. (C) Peptide sequences synthesized and the position of their spots. (D) Molecular modeling of CRA protein predicted by I-Tasser server. The visualization of the proteins was executed as a “new cartoon” representation with the quality set at the maximum value (50). The red segments are overlapping epitopes with the greatest signals contrasted against the rest of repeated structure in blue color.