Figure 2

Phylogenetic analysis of full-length HBV sequences. The neighbour-joining tree was generated with the MEGA5 software package [38] and shows the relationship between three novel, full-length HBV-G strains from the Netherlands (indicated by red circles) and reference sequences of HBV genotypes A1, A2, C, D1, D2 and G obtained from the NCBI nucleotide database [35]. Distances were estimated with the Kimura 2-parameter method, and bootstrap resampling was done with 1000 replicates. The accession number of the reference sequences is indicated together with the HBV genotype.