|
Inocula
|
Animal ID
|
ALT
|
AST
|
GGT
|
Tb
|
**Liver injury
|
HEV RNA detection
|
***anti-HEV
|
Clinical interpretation****
|
|---|
|
(IU/dL) (#maximum value obtained)
|
(Score)
|
(dpi*)
|
(EIA reactivity)
|
|---|
|
Serum
|
Saliva
|
|---|
|
Serum
|
Faeces
|
IgM
|
IgG
|
IgA
|
IgA
|
|---|
|
Br-swine
|
X15
|
73
|
295
|
315
|
0.8
|
2
|
---
|
14 - 21
|
+
|
+
|
+
|
nt
|
Subclinical hepatitis E
|
|
Q11
|
24
|
31
|
64
|
0.7
|
1
|
14
|
7 - 21
|
+
|
+
|
+
|
-
|
Subclinical hepatitis E without biochemical disorders
|
|
I3
|
40
|
54
|
224
|
0.8
|
2
|
39 - 53
|
21 - 53
|
-
|
-
|
-
|
+
|
Subclinical hepatitis E
|
|
D-swine
|
01
|
40
|
119
|
237
|
0.8
|
1
|
14
|
5 - 18
|
+
|
+
|
+
|
-
|
Subclinical hepatitis E
|
|
G3
|
32
|
42
|
138
|
0.8
|
1
|
7 - 14
|
7 - 18
|
+
|
+
|
+
|
+/-
|
Subclinical hepatitis E without biochemical disorders
|
|
F3
|
99
|
302
|
238
|
0.7
|
1
|
---
|
---
|
+
|
+
|
+
|
+
|
Biochemical and histological hepatitis E
|
|
Br-human
|
J3
|
57/46
|
50
|
293
|
0.8
|
1
|
---
|
---
|
-
|
+
|
-
|
-
|
Biochemical and histological hepatitis E
|
|
Ar-human
|
R7
|
21
|
213
|
188
|
0.8
|
0
|
---
|
14 - 27
|
+
|
+
|
+
|
nt
|
Subclinical hepatitis E
|
|
Control
|
I2
|
21
|
43
|
92
|
0.8
|
0
|
---
|
---
|
-
|
-
|
-
|
nt
|
Uninfected
|
|
Q12
|
21
|
116
|
103
|
0.7
|
0
|
---
|
---
|
-
|
-
|
-
|
-
|
Uninfected
|
- *dpi: days post inoculation.
- **Liver sections were prepared from large liver samples collected during necropsy; liver injury score (adapted from [29]): 0 – without inflammation, 1 – 1 to 2 lymphohistiocytic inflammatory infiltrates/10 liver lobules, 2–3 to 5 inflammatory infiltrates/10 lobules, 3–6 to 10 inflammatory infiltrates/10 lobules, 4- >10 inflammatory infiltrates/10 lobules (adapted from [29]).
- ***DiaCheck anti-human HEV antibody assay that was modified with a goat anti-macaque secondary antibody.
-
#The maximum value obtained during the entire follow-up period is provided.
- ****Subclinical hepatitis was considered when the animals presented only laboratorial finds of infection, Biochemical hepatitis was considered using species-specific normal values and pre-inoculation values. Liver enzymes values were elevated at least twice above baseline in the context of an HEV infection. Histological hepatitis was considered when the animals presented lymphohistiocytic inflammatory infiltrates in liver biopsy.
- Br: Brazilian, Ar: Argentinian, D: Dutch, EIA: Enzyme immunoassay, nt: not tested, (they did not produced enough saliva).
