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Figure 1 | BMC Infectious Diseases

Figure 1

From: First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood

Figure 1

Construction and characterization of spike expression plasmids derived from non-SARS HCoVs. (A) Construction scheme of spike (S) fragment expression plasmids derived from four individual HCoVs (−229E, -OC43, -NL63, and -HKU1). (B) Characterisation of S proteins of individual HCoV expression plasmids by western blot using anti-S mouse serum from DNA vaccination. Forty-eight hours after 293 T cell transfection with the above individual HCoV expression plasmids, the cell lysates were collected for SDS-PAGE and western blot. (C) Characterisation of S protein by indirect immune fluorescence assay (IFA) using anti-S serum from mice immunised with individual HCoV expression plasmids. The control was transfected with pVRC-8303 plasmid and detected with mixed anti-S mouse serum of a non-SARS CoV.

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BMC Infectious Diseases

ISSN: 1471-2334

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