Figure 2
From: Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification
Confirmation of RT-LAMP amplification of DENV. Restriction enzyme digestion of DENV-specific RT-LAMP-amplified DNA fragments were performed using BanII. The DNA fragments were separated on a 2% agarose gel. Lane M, 100-bp plus DNA ladder; lane 1, DENV-1 RT-LAMP assay amplification; lane 2, BanII digestion of DENV-1 RT-LAMP-amplified DNA fragments, 129Â bp and 233Â bp; lane 3, DENV-2 RT-LAMP assay amplification; lane 4, BanII digestion of DENV-2 RT-LAMP-amplified DNA fragments, 135Â bp and 231Â bp; lane 5, DENV-3 RT-LAMP assay amplification; lane 6, BanII digestion of DENV-3 RT-LAMP-amplified DNA fragments, 129Â bp and 231Â bp; lane 7, DENV-4 RT-LAMP assay amplification; lane 8, BanII digestion of DENV-4 RT-LAMP-amplified DNA fragments, 141Â bp and 231Â bp.