NLRC4 deficiency impairs replication in murine alveolar macrophages. Alveolar macrophages (AM) were isolated from lungs of WT (A), Nlrc4−/−
(B), and Tlr5−/−
(C) mice by bronchoalveolar lavage of uninfected mice. Cells were infected ex vivo with WT (Corby) Lp or isogenic FlaA- (Corby) Lp and CFUs were measured in lysed monolayers at 24, 48, and 72 hours. Data are mean ± SEM of triplicate or quadruplicate monolayers at each time point (except for duplicate measures at time 0 from TLR5−/− mice.). *p < 0.05, †p < 0.005 as determined by two-tailed Students’ t-test.