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Table 2 PCR and qPCR results for streptococcal reference isolates

From: Detection of group a streptococcal pharyngitis by quantitative PCR

  PCR assay* qPCR assay**
Species and strain speB(1) speB(2) parE spy1258(1) spy1258(2) spy1857 speB spy1258
ct value ct value
S. pyogenes IGL 1 + + - + + + 19.5 19.9
S. pyogenes IGL 6 + + - + + + 21.1 20.2
S. pyogenes IGL 13 + + - + + + 21.6 20.3
S. pyogenes IGL 165 + + - + + + 21.2 20.4
S. pyogenes IGL 181 + + +\- + + + 19.5 19.6
S. pyogenes ATCC BAA-572 + + +\- + + + 21.1 20.7
S. pyogenes IRP 187 + + - + + + 20.2 19.9
S. pyogenes 85RP187 + + + + + + 21.1 20.0
S. mitis PMP933 +\- +\- NS +\- +\- +\- No Ct No Ct
S. mitis PMP934 +\- +\- NS +\- +\- +\- No Ct 34.8
S. mitis PMP16 +\- +\- - +\- +\- +\- No Ct No Ct
S. pneumoniae ATCC 6305 - - + - - - No Ct No Ct
S. agalactiae ATCC 13813 - - NS - - + No Ct No Ct
S. agalactiae GBS78 +\- - NS - +\- + No Ct No Ct
S. agalactiae GBS79 - - - +\- +\- + No Ct No Ct
S. sanguis NTCT7864 - - NS - - - No Ct No Ct
S. mutans PMP935 - - NS - - + No Ct No Ct
  1. * + = strong PCR product at expected size; +/− = weak PCR product at expected size; NS = non-specific (PCR product at unexpected size and/or multiple PCR products); - = no PCR product.
  2. ** Cycle threshold (Ct) values are reported as the mean of duplicate wells containing 0.4 ng genomic DNA.