BMC Infectious Diseases

Table 2 PCR and qPCR results for streptococcal reference isolates

From: Detection of group a streptococcal pharyngitis by quantitative PCR

	PCR assay*						qPCR assay**
Species and strain	speB(1)	speB(2)	parE	spy1258(1)	spy1258(2)	spy1857	speB	spy1258
Species and strain	speB(1)	speB(2)	parE	spy1258(1)	spy1258(2)	spy1857	ct value	ct value
S. pyogenes IGL 1	+	+	-	+	+	+	19.5	19.9
S. pyogenes IGL 6	+	+	-	+	+	+	21.1	20.2
S. pyogenes IGL 13	+	+	-	+	+	+	21.6	20.3
S. pyogenes IGL 165	+	+	-	+	+	+	21.2	20.4
S. pyogenes IGL 181	+	+	+\-	+	+	+	19.5	19.6
S. pyogenes ATCC BAA-572	+	+	+\-	+	+	+	21.1	20.7
S. pyogenes IRP 187	+	+	-	+	+	+	20.2	19.9
S. pyogenes 85RP187	+	+	+	+	+	+	21.1	20.0
S. mitis PMP933	+\-	+\-	NS	+\-	+\-	+\-	No Ct	No Ct
S. mitis PMP934	+\-	+\-	NS	+\-	+\-	+\-	No Ct	34.8
S. mitis PMP16	+\-	+\-	-	+\-	+\-	+\-	No Ct	No Ct
S. pneumoniae ATCC 6305	-	-	+	-	-	-	No Ct	No Ct
S. agalactiae ATCC 13813	-	-	NS	-	-	+	No Ct	No Ct
S. agalactiae GBS78	+\-	-	NS	-	+\-	+	No Ct	No Ct
S. agalactiae GBS79	-	-	-	+\-	+\-	+	No Ct	No Ct
S. sanguis NTCT7864	-	-	NS	-	-	-	No Ct	No Ct
S. mutans PMP935	-	-	NS	-	-	+	No Ct	No Ct

* + = strong PCR product at expected size; +/− = weak PCR product at expected size; NS = non-specific (PCR product at unexpected size and/or multiple PCR products); - = no PCR product.
** Cycle threshold (Ct) values are reported as the mean of duplicate wells containing 0.4 ng genomic DNA.

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ISSN: 1471-2334

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