Table 1 Primers used in real-time PCR analysis of T.gondii IVIAT identified genes by IgM
GenBank ID/accession no. | Primer | |
|---|---|---|
| Â | Orientation sequence | |
** β -Actin | *F | 5′-TCACACTGTGCCCATCTACGA-3′ |
*R | 5′-GTGGTGAAGCCGTATCCTCTCT-3′ | |
7895406 | F | 5′-ACCGACTCGAAGCACAACAGT-3′ |
R | 5′-CATCGTAGGTGACGAACAATGC-3′ | |
7898829 | F | 5′- CAATGTTTCGGGCAGCTCAT -3′ |
R | 5′- GCTGTACTGAAACCGTCGATGTAG -3′ | |
7894091 | F | 5′- CCACTCAAGCAGTTCCTGAAGA -3′ |
R | 5′- GATCTCCGCGAAATCTGTCTCT -3′ | |
7900912 | F | 5′- CGCATTTACAAGCGCCTGAT -3′ |
R | 5′- TTCCACGCCTCGTTTACATG -3′ | |
7899945 | F | 5′- CTTCTCGACGACATGCATCTG -3′ |
R | 5′- TGGACGCACTGGATGTTCTG -3′ | |
7893976 | F | 5′- AGCGCAGCAAGAACCAAGA -3′ |
R | 5′- TTCCTCAAGAAGGCCATGGA -3′ | |
7893824 | F | 5′- CTCACGTACGAGGCTAGATCCA -3′ |
R | 5′- GTCCGCCAGGGCATTCTATT -3′ | |
7899621 | F | 5′- AGGCACAGGCTCCACCATAC -3′ |
R | 5′- AGCTCCAGGACGCGTAAGAG -3′ | |
7901413 | F | 5′- TCTGAAGCTGCAAGGTTCCA -3′ |
R | 5′- TTGCAGAGTAGGCTCGTCAAAC -3′ | |
7894150 | F | 5′- TCCTCGAACGGCAGAGAAA -3′ |
R | 5′- CCCCTCAGGTGTGAAGACATCT -3′ | |
7895338 | F | 5′- AGAGCCGGTAGCACTGTGTTC -3′ |
R | 5′- CGGTAACCCGGTGGACTTCT -3′ | |
7898197 | F | 5′- GGATTTCGGCACGCTCAAT -3′ |
R | 5′- CAACTGTGGCTCCCGACTACTT -3′ | |
7895974 | F | 5′-CCAAATCGGTTCGCTCATGT-3′ |
R | 5′-GCAAAGGCCGGAGAATTCAT-3′ | |
7900011 | F | 5′-CACGCTAACTGTTTCAGGTACGA-3′ |
R | 5′-TACAGCAGCGTGTTCATTTGC-3′ | |
7895000 | F | 5′-TCCAACATCCCGACCTGATC-3′ |
R | 5′-GCTTCGACCTTCGCATTCTTC-3′ | |
L06091.1 | F | 5′-CAGACTCCTTGCTCTCTTCTTGAA-3′ |
R | 5′-GTTGTGTCACTACTGCGATTGTTG-3′ | |
7896316 | F | 5′-AAGGTGCTTCATCCGCCATA-3′ |
R | 5′-GGTCTTGCATATCTCGCCATTC-3′ | |
7894239 | F | 5′-GAACTGTTTCAATGCGTCGATT-3′ |
R | 5′-GTGATCCAATCGCACACTCTTTT-3′ | |
7896580 | F | 5′-CCTTCTGGTTAGCCACATCTGAT-3′ |
R | 5′-GGCGACGAAGATGATTTTGAC-3′ | |
7899034 | F | 5′-CCTGGAACGTCCTTTCTGGAT -3′ |
R | 5′-ATCTAACCGTCGCGCTGGTA -3′ | |
7899034 | F | 5′-ATGTTCCCGATCCAGAGCAA-3′ |
R | 5′-GGTGTGGCTGTCTCCCTATTTT-3′ | |
AY707938.1 | F | 5′-GAACGCGAACAATGAGTTTG-3′ |
R | 5′-CCGCTGGTCGAAATAATGATG-3′ | |
7894324 | F | 5′-GTTAATCAGGAGCAGACCCGATA-3′ |
R | 5′-TAAGCTACCCCTGAGCAAGTTGT-3′ | |
AF364813.1 | F | 5′-GGTGGAAAGTGAATACCGTGGAT-3′ |
R | 5′-CAGTGAGTCCGAATGCCAAAT-3′ | |
7900910 | F | 5′-GCCTGCAACATGTCCAACTTC-3′ |
R | 5′-TCAGCGGCTAAGCGAAAAAG-3′ | |
7896316 | F | 5′-CATACGGATAGGCGGTCATGTA-3′ |
R | 5′-GTATACGAACGCCCTGATGTTG-3′ | |
7897539 | F | 5′-ATGCAAGACCCAAGGCAAAC-3′ |
R | 5′-TGTACGGAACGCTTCTTCGA-3′ | |
7895127 | F | 5′-GCGAAAGTCAAACGGAATGG-3′ |
R | 5′-TTCTGCGCGGAGTCGTTATT-3′ | |
7896796 | F | 5′-GCTTTTACCTGCTCGGGAAGT-3′ |
|  | R | 5′-AACGCATCTCACACGGATAGC-3′ |