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BMC Infectious Diseases

Open Access

Detection of Salmonella enteritidis, typhi and typhimurium in foods by multiplex PCR in children hospital

  • Shima Nosraty1,
  • Fatemeh Fallah2Email author,
  • Azar Sabokbar1,
  • Mehrooz Dezfoolyan1,
  • Saadat Adabian1 and
  • Narges Esmaeilnejad1
BMC Infectious Diseases201212(Suppl 1):P95

Published: 4 May 2012


Public HealthInternal MedicineInfectious DiseaseQuality ControlFood Item


Food-borne illnesses have high prevalence in the world, which are caused by consumption of water or food contaminated. Nowadays, involvement caused by Salmonella bacterium, serotype enteritidis and typhimurium are the major infection all around the world. So the purpose of this study is detection of Salmonella enteritidis, typhi and typhimurium in different kinds of foods by multiplex PCR method.


The study was conducted on 170 samples of food products including, milk, beef, poultry, salad dressing and 80 samples in contact with food items such as knives, cutting boards and the hands of personnel working in a hospital’s kitchen. After collecting the samples, the standard diagnostic method for detection of bacteria were performed and after extracting the DNA, the multiplex PCR method has been used for determination of Salmonella serotypes.


From all of the tested food samples (170), 1.7% of Salmonella contamination has observed that 1.1% related to typhimurium and 0.6% related to Salmonella enteritidis that had separated from beef, and during the tests conducted on samples that were in contact with food items, Salmonella contamination was not detected.


Results indicated that, quality control of food products in processing and production stages observing hygiene issues are critically important in preventing food-borne diseases. And also the methods like molecular diagnostic such as multiplex PCR besides the cultivation of bacteria and other microbes can be helpful in diagnostic confirmation.

Authors’ Affiliations

Karaj Branch, Islamic Azad University, Karaj, Iran
Pediatric Infectious Research Center, Shahid Beheshti Medical University, Tehran, Iran


© Nosraty et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.