Volume 12 Supplement 1

Abstracts from the First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)

Open Access

Antitubercular drugs induced hepatic oxidative stress and ultrastructural changes in rats

BMC Infectious Diseases201212(Suppl 1):P85

https://doi.org/10.1186/1471-2334-12-S1-P85

Published: 4 May 2012

Background

As the adverse side effect hepatotoxicity, constitutes an essential part of antituberculosis chemotherapy, the present study was designed to investigate the pathogenesis of antituberculosis (anti-TB) drugs induced hepatic effects in rats with the first-line treatment regimen of isoniazid, rifampicin and pyrazinamide.

Methods

The rats were divided into three groups (n=6 per group), group I served as a control, group II received orally combination of isoniazid (15mg/kg body weight), rifampicin (20mg/kg body weight) and pyrazinamide (35mg/kg body weight) daily for 45 days and group III received simultaneously Silymarin (50mg/kg body weight) and combination of anti-TB drugs at the above mentioned dosages for 45 days. After the experimental period, the levels of malondialdehyde (MDA, oxidative stress marker) and lipid profile was evaluated in serum. The data were analyzed by Duncan’s multiple range tests. The pathological and morphological changes were examined histologically and electron microscopically.

Results

The rats administered anti-TB drugs alone, showed a significantly increase in serum MDA levels and lipid profile (p<0.001). Histopathological features of group II rats showed inflammatory cell infiltration and spotty necrosis. The electron micrograph results indicated kupffer cell hyperplasia, swollen mitochondria and loss of cell architecture. Co-administration of Silymarin significantly decreased anti-TB drugs-induced changes in serum MDA levels, lipids (p<0.001) and retained the liver integrity.

Conclusions

The anti-TB drugs can induce hepatic oxidative stress and the level of serum MDA may be a more sensitive biomarker for monitoring drug-induced hepatotoxicity. Hepatoprotective compounds with antioxidant potential can be supplemented to prevent anti-TB drugs induced cellular oxidative stress.

Authors’ Affiliations

(1)
Department of Biomedical Science, Bharathidasan University
(2)
Department of Biochemistry, University of Madras

Copyright

© Saraswathy and Devi; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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