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In vitro anti-HIV activity of crude extracts from Tinospora cordifolia
© Estari et al; licensee BioMed Central Ltd. 2012
- Published: 4 May 2012
- Human Immunodeficiency Virus
- Crude Extract
- Acquire Immune Deficiency Syndrome
- Ethyl Acetate Extract
- Peripheral Mononuclear Blood
Human immunodeficiency virus (HIV) infection causes acquired immune deficiency syndrome (AIDS) and is a global public health issue. Anti-HIV therapy involving chemical drugs has improved the life quality of HIV/AIDS patients. However, emergence of HIV drug resistance, side effects and the necessity for long-term anti-HIV treatment are the main reasons for failure of anti-HIV therapy. Therefore, it is essential to isolate novel anti-HIV therapeutics from natural resources. The aim of the present study was to evaluate the invitro anti-HIV activity of T. cordifolia plant extracts.
Extracts were prepared from dried leaves in n-hexane, dichloromethane, ethyl acetate and n-butanol. A toxicity study was performed on all crude extracts using peripheral mononuclear blood cells (PBMCs) isolated from whole blood. HIV-1 RT inhibition activity of the all solvent extracts of T. cardifolia was determined using a commercial kit.
Among the tested extracts, the n-hexane and n-butanol crude extracts of T. cordifolia showed moderate cytotoxic activities against PBMCs with CC50 values ranging from 5.7-12.0 µg/ml. In the HIV-1 reverse transcriptase assay T. cardifolia plant extracts showed good inhibitory activity, which was near that of the reference drug. Ethyl acetate extract shows 85 percentage of HIV-1 RT inhibition activity at a concentration of 20 mg/ml.
The leaves of T. cardifolia extracts are shows anti-HIV 1 activity and this plant has great potential for developing useful drugs. Extraction of important biologically-active phytochemicals from this plant will certainly be helpful in protecting and treating various viral diseases in human beings.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.