Figure 4

CXCL9 production by infected human macrophages cultured in vitro in the presence of L. braziliensis. Six-day monocyte derived-macrophages from HS (n = 14), SC (n = 12), CL (n = 16), and ML (n = 11) were infected in vitro with L. braziliensis at a 2:1 ratio. After 48 h, the supernatants were harvested and the levels of CXCL9 were determined by ELISA. Each symbol represents a different patient, and each line represents the median of a group. For statistical analysis, the Kruskal-Wallis test was used. (*p < 0.05; ***p < 0.001).