Figure 2

Inter-group proliferation analysis of T-cell subpopulations. Carboxyfluorescein diacetate succinimidyl ester (CFSE) flow cytometry was performed to assess the responsiveness of CD4⁺ and CD8⁺ cells after 120 hours of culture. PBMCs were stimulated with soluble egg antigen (SEA) and soluble worm antigen preparation (SWAP). The mitogen controls (phytohemagglutinin - PHA) of the CD4⁺ and CD8⁺ T cells are presented (G and H). The results are expressed as the median (interquartile range) for each group BD: n = 19, NI: n = 31 and XTO: n = 44. The egg counts in the XTO group ranged from 16 to 384 eggs/g of stool (mean = 200 eggs/g). The Kruskal-Wallis and Dunn's post-test were performed for statistical analysis. The letter "a" represents the difference (p <0.05) compared to BD group.