Figure 3

EV71-induced apoptosis in RD cells. Early membrane revealed an obvious change associated with apoptosis in EV71-infected RD cells. The exposure of phosphatidylserine (PS) was analyzed using FITC-labeled annexin V. Cell death was assessed with propidium iodide (PI). EV71-infected RD cells were harvested at 20 and 32 h postinfection and stained with annexin V-FITC/PI, and then observed under a fluorescence microscope (400×).