Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Table 1 Evaluation of r-Δgp160-based TRF assays using HIV-1 seroconversion panel (PRB 931)

Member ID#	Days since 1^stbleed	Abbott		Gen. Sys.		Organon Teknika HIV^a	TRF assays^b
Member ID#	Days since 1^stbleed	HIV1^a	HIV1/2^a	HIV1^a	HIV1/2^a	Organon Teknika HIV^a	r-Δgp160 two-step	r-Δgp160 one-step
01	0	0.2	0.1	0.2	0.1	0.3	0.9 (−)	0.5 (−)
02	2	0.2	0.1	0.1	0.1	0.3	0.8 (−)	0.5 (−)
03	7	0.2	0.1	0.2	0.1	0.4	0.8 (−)	0.4 (−)
04	9	0.2	0.1	0.2	0.1	0.3	0.8 (−)	0.5 (−)
05	15	0.2	0.1	0.2	0.1	0.3	0.7 (−)	0.5 (−)
06	28	0.9	6	0.3	0.4	0.6	17.2 (+)	5.6 (+)
07	33	3.9	>18.7	0.8	1.1	2.3	15.9 (+)	4.3 (+)
08	35	5.7	>18.7	1.3	1.9	3.1	11.9 (+)	3.3 (+)
09	42	10.5	>18.7	2.9	4	4.6	13.8 (+)	2.9 (+)

^aValues indicate S/Co ratios, provided by the panel supplier using the indicated commercial EIA kits. S/Co values ≥ 1.0 are considered as positive.
^bValues indicate S/Co ratios obtained using r-Δgp160-based TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+).

ISSN: 1471-2334