In vitroantimalarial susceptibility and molecular markers of drug resistance in Franceville, Gabon

BMC Infectious Diseases

Table 1 Sequences of primer sets and restriction enzymes used to characterized polymorphisms

Genes, Codons	Primer names	Primers	T°C	Restriction enzyme	sizes (bp)*
*Pfmdr1, N86Y*	mdr86D1	TTTACCGTTTAAATGTTTACCTGC	45	*Afl III*	126 +165
*Pfmdr1, N86Y*	mdr86D2	CCATCTTGATAAAAAACACTTCTT	45	*Afl III*	126 +165
*Pfmdr1, D1246Y,*	mdr1246D1	AATGTAAATGAATTTTCAAACC	45	*Bgl II*	113 + 90
*Pfmdr1, D1246Y,*	mdr1246D2	CATCTTCTCTTCCAAATTTGATA	45	*Bgl II*	113 + 90
*ATPase 6, G110A*	ATP6-110F	CGTTGAACTTATTATATCTTTGTC	60	*Mbo II*	103+94+92+40
*ATPase 6, G110A*	ATP6-110R	TTTCATATCTAATAAAGTTAACACG	60	*Mbo II*	103+94+92+40
*ATPase 6, A2694T*	ATP6-2694F	GAATTGTTTTCTGTAGAACTGAAC	55	*Tas I*	142 + 39
*ATPase 6, A2694T*	ATP6-2694R	ATCTGATGCTTCTTTAGCTACC	55	*Tas I*	142 + 39
*Pfcrt, C72S*	CRT72MS	TTTATATTTTAAGTATTATTTATTTAAGTGGA	55	*Mbo I*	55 + 38
*Pfcrt, C72S*	76-D2	CAAAACTATAGTTACCAATTTTG	55	*Mbo I*	55 + 38
*Pfcrt, M74I*	CRT745MS	TAAGTATTATTTATTTAAGTGTATGTGTCAT	55	*Nla III*	53 + 31
*Pfcrt, M74I*	76-D2	CAAAACTATAGTTACCAATTTTG	55	*Nla III*	53 + 31
*Pfcrt, N75M*	CRT745MS	TAAGTATTATTTATTTAAGTGTATGTGTCAT	50	*BspHI*	53 + 31
*Pfcrt, N75M*	76-D2	CAAAACTATAGTTACCAATTTTG	50	*BspHI*	53 + 31
*Pfcrt, 76*	Pfcrt-76A	GCGCGCGCATGGCTCACGTTTAGGTGGAG	55	*Apo I*	136 + 56
*Pfcrt, 76*	Pfcrt-76B	GGGCCCGGCGGATGTTACAAAACTATAGTTACC	55	*Apo I*	136 + 56

sizes* indicate the sizes of fragments generated after restriction enzyme digestions.
AA¹ = mutation on amino acid sequence.
T°C = hybridization temperature during PCR programme.

ISSN: 1471-2334