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Table 4 Comparison of the LAMP assay with nested PCR assay for the detection of the spotted fever rickettsiae group in clinical samples a

From: Rapid, simple, and sensitive detection of the ompB gene of spotted fever group rickettsiae by loop-mediated isothermal amplification

No. of cases Diagnosis type LAMP Nested PCR Real-time PCR
1 gltA gene amplified Pos. Neg. Pos.
2 gltA gene amplified Pos. Neg. Pos.
3 4-fold IgG titer increase Pos. Neg. Neg.
4 Culture confirmed and gltA gene amplified Pos. Neg. Pos.
5 4-fold IgG titer increase Pos. Neg. Neg.
6 4-fold IgG titer increase and gltA gene amplified Pos. Neg. Pos.
7 4-fold IgG titer increase and gltA gene amplified Pos. Neg. Pos.
8 Suspected case Neg. Neg. Pos.
9 Culture confirmed Neg. Neg. Neg.
10 4-fold IgG titer increase Neg. Neg. Neg.
11 4-fold IgG titer increase and gltA gene amplified Pos. Neg. Pos.
  1. a All assays were performed in triplicate.