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Table 1 Oligonucleotide primers and hydrolysis probes used in this study

From: Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis

Primers or Probes Sequence Gene Size
Primer    
JP2-F3 5’-TCT ATG AAT ACT GGA AAC TTG TTC AGA AT-3’ orfX’ 151 bpd
JP2-R2 5’-GAA TAA GAT AAC CAA ACC ACA ATA TCC-3’ orfX’
Non-JP2F 5’-CGC AAG TGC CAT AGT TAT CCA CT-3’ orfX (∆530 region) 145 bpe
Non-JP2R 5’-TCG TCT GCG TAA TAA GCA AGA GAG-3’ orfX (∆530 region)
Aa orfX3’F1 5’-AAA TCG TTG GCA TTC TCG -3’ orfX 723 bpe
Aa lktC5’R1 5’-CAA AGG AGA ATT TGC CCA -3’ lktC
Aa orfX3’F2 5’-CCG TTT TAT TCA GTT CCC -3’ orfX 809 bpe
Aa lktC5’R2 5’-TGC CCA TAA CCA AGC CAC -3’ lktC
Hydrolysis probesa, b, c    
JP2 5’-FAM-ACA AAT CGT TGG CAT TCT CGG CGA A-TAMRA-3’ orfX’  
Non-JP2 5’-FAM-ATA TTG TAG ACA TCG CCC-MGB-3’ orfX (∆530 region)  
  1. aFAM, 6-carboxyfluorescein.
  2. bTAMRA, 6-carboxytetramethylrhodamine.
  3. cMGB, minor groove binder.
  4. dcalculated in JP2 strain.
  5. ecalculated in Y4 strain.