Evaluation of the antiviral effects of Lenti-2B in HeLa cells.
A HeLa cells were transduced with Lenti-2B at 1, 10, 100 TU/ml, respectively, or Lenti-NC or MEM only (V), followed by CVB3 infection (MOI = 0.01). The viral titers were measured at 48 h p.i. using supernatants of cell cultures by plaque-forming analysis and the results of three independent experiments are expressed as mean ± standard deviation. Significant differences between Lenti-2B treated (white bars) and Lenti-NC treated samples (striped bars) are indicated (*: P < 0.05) B HeLa cells were transduced with Lenti-2B (10 TU/well) or Lenti-NC or MEM only (Virus), followed by CVB3 infection (MOI = 0.01). After 48 h, GFP-expressing cells were visualized by fluorescence microscopy. LM: light microscopy, FM: fluorescence microscopy.