Figure 4
Comparison of PCR sensitivities between the DNA preparations. Salmonella serovar Typhi fliC-d amplicons (763 bp) amplified by PCR using different DNA preparations were separated on a 1% agarose gel. M: DNA marker; P: Salmonella Typhi DNA positive control; N: No DNA negative control; Lanes 1–7: PCR on DNA prepared by STEM1; Lanes 8–14; PCR on DNA prepared by the conventional method. Lanes 1 and 8, 2 and 9, 3 and 10, 4 and 11, 5 and 12, 6 and 13, 7 and 14: DNA prepared from the spiked blood samples containing 3×105, 3×104, 3×103, 3×102, 30, 3, 0 CFU/ml of Salmonella Typhi, respectively.