Table 5 Comparison of RVP, RespiFinder-19 and RespiFinder-SMART-22 with regard to test specifications, performance, cost and accomplishment
| Â | RVP | RespiFinder-19 | RespiFinder-SMART-22 |
|---|---|---|---|
Manufacturer | Abbott Molecular | PathoFinder | PathoFinder |
CE IVD/FDA | yes/yes | yes/no | yes/no |
TEST SPECIFICATIONS | Â | Â | Â |
Pathogens | influenza A + B | influenza A + B | influenza A + B |
|  | RSV-A + B | RSV-A + B | RSV-A + B |
| Â | adenovirus | adenovirus | adenovirus |
| Â | hMPV | hMPV | hMPV |
| Â | PIV-1, -2, -3, -4 | PIV-1, -2, -3, -4 | PIV-1, -2, -3, -4 |
| Â | CoV 229E, NL63, OC43, HKU1 | CoV 229E, NL63, OC43 | CoV 229E, NL63, OC43, HKU1 |
| Â | HRV | HRV | HRV |
| Â | enterovirus | - | enterovirus |
| Â | bocavirus | - | bocavirus |
| Â | - | B. pertussis | B. pertussis |
| Â | - | C. pneumoniae | C. pneumoniae |
| Â | -- | L. pneumophilaM. pneumoniae | L. pneumophilaM. pneumoniae |
Sample volume | 500 μL | 200 μL | 200 μL |
Elution volume | 55 μL | 100 μL | 100 μL |
PCR volume | 10 μL | 10 μL | 10 μL |
Principle of detection | fluorescence bead array | capillary electrophoresis | melt curve analysis |
PERFORMANCE | Â | Â | Â |
Hands-on-time | 0.80 h | 1.75 h | 1.25 h |
Time-to-result | 3.5 h | 7.5 h | 6.0 h |
COSTS | Â | Â | Â |
Costs reagents * ,1 | 90-100 € | 50-60 € | 50-60 € |
Costs equipment * | 57-69,000 € | 100-120,000 € | 33-40,000 € |
ACCOMPLISHMENT | Â | Â | Â |
Handling | + | + | ++ |
Personal training duration/qualification | 2 d/very low | 2 d/very low2 | 2 d/low |
Technical equipment | extraction system, thermocycler, Luminex 200 | extraction system, thermocycler, DNA-Sequencer ABI3500 | extraction system, thermocycler, Rotorgene Q |