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Table 5 Comparison of RVP, RespiFinder-19 and RespiFinder-SMART-22 with regard to test specifications, performance, cost and accomplishment

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

  RVP RespiFinder-19 RespiFinder-SMART-22
Manufacturer Abbott Molecular PathoFinder PathoFinder
CE IVD/FDA yes/yes yes/no yes/no
TEST SPECIFICATIONS    
Pathogens influenza A + B influenza A + B influenza A + B
  RSV-A + B RSV-A + B RSV-A + B
  adenovirus adenovirus adenovirus
  hMPV hMPV hMPV
  PIV-1, -2, -3, -4 PIV-1, -2, -3, -4 PIV-1, -2, -3, -4
  CoV 229E, NL63, OC43, HKU1 CoV 229E, NL63, OC43 CoV 229E, NL63, OC43, HKU1
  HRV HRV HRV
  enterovirus - enterovirus
  bocavirus - bocavirus
  - B. pertussis B. pertussis
  - C. pneumoniae C. pneumoniae
  -- L. pneumophilaM. pneumoniae L. pneumophilaM. pneumoniae
Sample volume 500 μL 200 μL 200 μL
Elution volume 55 μL 100 μL 100 μL
PCR volume 10 μL 10 μL 10 μL
Principle of detection fluorescence bead array capillary electrophoresis melt curve analysis
PERFORMANCE    
Hands-on-time 0.80 h 1.75 h 1.25 h
Time-to-result 3.5 h 7.5 h 6.0 h
COSTS    
Costs reagents * ,1 90-100 € 50-60 € 50-60 €
Costs equipment * 57-69,000 € 100-120,000 € 33-40,000 €
ACCOMPLISHMENT    
Handling + + ++
Personal training duration/qualification 2 d/very low 2 d/very low2 2 d/low
Technical equipment extraction system, thermocycler, Luminex 200 extraction system, thermocycler, DNA-Sequencer ABI3500 extraction system, thermocycler, Rotorgene Q
  1. *All prices are for Germany (list price).
  2. 1Prices for one sample.
  3. 2The personal training will be very low, if technicians are familiar with sequencing using an ABI system.