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Table 1 Key parameters for monoplex real-time PCR assays

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

Virus

Dynamic range

Efficiency

Interassay- variabilty

R2

INF-A H1

1.00E + 05 - 1.00E + 01

1.89

22.7 ± 0.49

0.972

INF-A H3

1.00E + 05 - 1.00E + 01

1.89

22.7 ± 0.49

0.972

RSV-A

1.00E + 06 - 1.00E + 01

1.98

22.4 ± 0.36

0.998

PIV-1

1.00E + 06 - 1.00E + 01

1.91

29.9 ± 0.84

0.993

PIV-2

1.00E + 05 - 1.00E + 01

1.95

29.3 ± 0.33

0.997

PIV-3

1.00E + 06 - 1.00E + 01

1.88

36.4 ± 0.30

0.989

HRV

1.00E + 06 - 1.00E + 01

1.90

21.3 ± 0.34

0.991

AdV

1.00E + 06 - 1.00E + 01

1.88

19.9 ± 0.17

0.989

hMPV

1.00E + 06 - 1.00E + 01

1.87

26.7 ± 0.66

0.986

  1. *Concentration of viruses are concordant to Table 2.