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Table 1 Key parameters for monoplex real-time PCR assays

From: Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay

Virus Dynamic range Efficiency Interassay- variabilty R2
INF-A H1 1.00E + 05 - 1.00E + 01 1.89 22.7 ± 0.49 0.972
INF-A H3 1.00E + 05 - 1.00E + 01 1.89 22.7 ± 0.49 0.972
RSV-A 1.00E + 06 - 1.00E + 01 1.98 22.4 ± 0.36 0.998
PIV-1 1.00E + 06 - 1.00E + 01 1.91 29.9 ± 0.84 0.993
PIV-2 1.00E + 05 - 1.00E + 01 1.95 29.3 ± 0.33 0.997
PIV-3 1.00E + 06 - 1.00E + 01 1.88 36.4 ± 0.30 0.989
HRV 1.00E + 06 - 1.00E + 01 1.90 21.3 ± 0.34 0.991
AdV 1.00E + 06 - 1.00E + 01 1.88 19.9 ± 0.17 0.989
hMPV 1.00E + 06 - 1.00E + 01 1.87 26.7 ± 0.66 0.986
  1. *Concentration of viruses are concordant to Table 2.