Figure 4

Proinflammatory cytokine levels in culture supernatants of Chandipura virus-infected RAW cells. RAW cells were infected with virus. The supernatant was collected on alternate hours up to 10 h PI. The final sample was collected 16 h PI. The levels of TNF-α, MCP-1, IL-10, IL-6, IL-12 and IFN-γ in the undiluted supernatant were quantified using a cytometric bead assay. The beads were acquired and analyzed using a FACSCalibur cell sorter and BD™ Cytometric Bead Array Software (BD Bioscience). The level of cytokines was quantified using known standard supplied with kit. Values represent the mean + standard error (SE) of triplicate experiments. IFN-γ could not be detected in the samples.