Figure 1From: Community-based cross-sectional survey of latent tuberculosis infection in Afar pastoralists, Ethiopia, using QuantiFERON-TB Gold In-Tube and tuberculin skin test The trend of proportion of positivity by QFTGIT against the skin test indurations. Whole blood samples were collected from study participants directly into tubes containing TB-specific antigens, mitogen and nil. The samples were incubated for 24 hours at 37°C, plasma was collected and IFNγ assay was performed using ELISA. The results were interpreted as positive, negative or indeterminate on the basis of the manufacturer's recommended cut-off value (IFN-γ ≥ 0.35 IU/ml) using QFTGIT analysis software developed by the company. Tuberculin skin test (TST) was also performed by administrating 0.1 ml (2T.U/0.1 ml) tuberculin PPD RT23 (Statens Serum Institute, Copenhagen, Denmark) intradermally in the middle of the left forearm. The diameter of the indurations was measured transversely after 48-72 h using a ball-point pen and flexible plastic ruler. The subjects were categorized in to three groups based on the size of skin test induration (0 mm, >0 < 10 mm and ≥ 10 mm) and the positivity by QFTGIT was compared for the three groups.Back to article page