IS element IS16 as a molecular screening tool to identify hospital-associated strains of Enterococcus faecium

BMC Infectious Diseases

Table 2 Localization of IS16 to I-Ceu-I digested chromosomal DNA resolved in PFGE and plasmid DNA as based on Southern hybridizations.

Strain no.	Origin	MLST type	*van*genotype	I-*Ceu*I PFGE	Plasmid
UW 6714	BC	202	-	-*	+
UW 1245	BC	202	vanA	-	+
UW 6293	BC	202	vanB	-*	-^#
UW 6295	BC	202	vanB	-*	-^#
UW 4962	BC	203	vanA	+	-^#
UW 6989	BC	203	-	+	-^#
UW 6502	BC	208	vanB	+	+
UW 5918	BC	252	vanA	+	+
UW 4675	BC	252	vanB	+	-
UW 6617	BC	275	vanA	-	+
UW 4960	BC	279	vanB	+	-
UW 6033	BC	282	vanA	+	+
UW 4512	BC	294	-	+	+
UW 6297	BC	306	-	+	+
UW 6935	BC	371	-	+	+
UW 3142	BC	375	vanA	+	+
UW 5275	BC	376	vanB	+	+
UW 6983	BC	377	-	+	+
UW 6112	BC	378	-	+	+
UW 4208	BC	New	-	+	+
UW 6642	BC	New	vanA	+	+
AK-EM 40	HC	18	-	+	+
AK-EM 53	HC	413	-	+	+
K226.7	HC	18	-	+	-
UW 2055	BC	16	vanA	-*	+
UW 3695	BC	17	-	+	+
UW 3544	BC	17	vanA	+	-^#
UW 5352	BC	17	vanB	-	+
UW 6990	BC	18	-	+	+
UW 1952	BC	18	vanA	+	+
UW 2900	BC	18	vanB	+	-^#
UW 1218	BC	19	vanA	+	-^#
UW 3056	BC	65	-	+	+
UW 6880	BC	78	-	+	+
UW 4671	BC	78	vanA	+	-
UW 2457	BC	80	vanA	+	-
UW 6985	BC	81	-	-	+
UW 2771	BC	81	vanA	+	-
UW 6882	BC	117	-	+	-
UW 1983	BC	117	vanA	+	-
UW 6805	BC	125	vanA	+	+
UW 2824	BC	132	vanA	+	-^#
UW 1716	BC	192	-	+	-^#
UW 5852	BC	192	vanA	+	+
UW 5267	BC	192	vanB	+	-^#

If available, different representatives of identical MLST types were considered (see for instance ST17 vanA/B/-negative isolates).
PFGE, pulsed-field gel electrophoresis; MLST, Multi-locus sequence typing; BC, blood culture; HC, human commensal; *, Hybridization signal with PFGE slot; ^#, negative results in repeated experiments.

ISSN: 1471-2334