Effect of targeting the Tim-3 and PD-1 signaling pathways on
-specific humoral and cellular responses. (A) Serum IgG antibodies from both groups (open squares for control and filled squares for anti-Tim-3 + PD-L1 treatment) were titrated using C. muridarum-infected HeLa cells as antigens under an immunofluorescence assay. The Log10 dilution was used to calculate the mean and standard deviation from each group as displayed along the Y-axis and at a given time along the infection time course (X-axis). There was no statistic difference in antibody titers between the two groups at any time points (ANOVA). (B) A total of 32 mouse cytokines were measured (using the Bio-Rad Bio-Plex kits) from the supernatants of splenocytes harvested from either control or antibody-treated groups were in vitro restimulated with medium alone (open bar) or UV-inactivated C. muridarum EBs (solid bar) as indicated along the X-axis. The cytokine concentrations from each group and restimulation condition were calculated in pg or ng/ml as mean and standard deviation as displayed along the Y-axis. Only the cytokines representing Th2 (IL-5, panel a), Th17 (IL-17, b) and Th1 (IFNg, c) were shown and the other 29 cytokines were not shown. There was no statistic difference in any of the 32 cytokine concentrations between the two groups.