Skip to main content

Advertisement

Figure 4 | BMC Infectious Diseases

Figure 4

From: Enhanced upper genital tract pathologies by blocking Tim-3 and PD-L1 signaling pathways in mice intravaginally infected with Chlamydia muridarum

Figure 4

Effect of targeting the Tim-3 and PD-1 signaling pathways on C. muridarum -specific humoral and cellular responses. (A) Serum IgG antibodies from both groups (open squares for control and filled squares for anti-Tim-3 + PD-L1 treatment) were titrated using C. muridarum-infected HeLa cells as antigens under an immunofluorescence assay. The Log10 dilution was used to calculate the mean and standard deviation from each group as displayed along the Y-axis and at a given time along the infection time course (X-axis). There was no statistic difference in antibody titers between the two groups at any time points (ANOVA). (B) A total of 32 mouse cytokines were measured (using the Bio-Rad Bio-Plex kits) from the supernatants of splenocytes harvested from either control or antibody-treated groups were in vitro restimulated with medium alone (open bar) or UV-inactivated C. muridarum EBs (solid bar) as indicated along the X-axis. The cytokine concentrations from each group and restimulation condition were calculated in pg or ng/ml as mean and standard deviation as displayed along the Y-axis. Only the cytokines representing Th2 (IL-5, panel a), Th17 (IL-17, b) and Th1 (IFNg, c) were shown and the other 29 cytokines were not shown. There was no statistic difference in any of the 32 cytokine concentrations between the two groups.

Back to article page