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Table 1 Characteristics of FISH probes used for hybridisation studies

From: Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens

Name Target use dye Sequence (5'-3') Tm (°C)
EUK 516 18S pos 5'-Cy3 ACC AGA CTT GCC CTC C 59.3
Univ. SSU 18S pos 3'-FAM GAC GGG CGG CGG TGT GTA CAA 58.3
Non 338 n.a. neg 3'-Cy3 ACT CCT ACG GGA GGC AGC 59.5
Prev 16S neg 3'-FITC CCA CAT GTT CCT CCG CTT GT 59.4
D223 28S broad 5'-Cy3 CCA CCC ACT TAG AGC TGC 62,2
D223IO 28S broad 5'-Cy3 CCA CCC GCT TGG AGC TG 55.8
Asp F 28S Asp 5'-FITC TGA CGG CCC GTT CCA G 61.8
Cand 317 28S C.a.,C.g 5'-FITC CAC GTA CTT TTT CAC TCT C 55.8
  1. Pos: positive Control, neg: Negative Control, broad: Broad spectrum fungal, Asp: Aspergillus group, C.a.: Candida albicans group, C.g.: C. glabrata