Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens

Table 1 Characteristics of FISH probes used for hybridisation studies

Name	Target	use	dye	Sequence (5'-3')	Tm (°C)
EUK 516	18S	pos	5'-Cy3	ACC AGA CTT GCC CTC C	59.3
Univ. SSU	18S	pos	3'-FAM	GAC GGG CGG CGG TGT GTA CAA	58.3
Non 338	n.a.	neg	3'-Cy3	ACT CCT ACG GGA GGC AGC	59.5
Prev	16S	neg	3'-FITC	CCA CAT GTT CCT CCG CTT GT	59.4
D223	28S	broad	5'-Cy3	CCA CCC ACT TAG AGC TGC	62,2
D223IO	28S	broad	5'-Cy3	CCA CCC GCT TGG AGC TG	55.8
Asp F	28S	Asp	5'-FITC	TGA CGG CCC GTT CCA G	61.8
Cand 317	28S	C.a.,C.g	5'-FITC	CAC GTA CTT TTT CAC TCT C	55.8

Pos: positive Control, neg: Negative Control, broad: Broad spectrum fungal, Asp: Aspergillus group, C.a.: Candida albicans group, C.g.: C. glabrata

ISSN: 1471-2334