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Figure 6 | BMC Infectious Diseases

Figure 6

From: Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens

Figure 6

Difficulties in the evaluation of hybridisation signals in samples with mould infection. Autofluoresence of hyphae (a-d) or surrounding tissue (e-h) in two tissue specimens from patients with invasive aspergillosis. Hybridisation with DAPI and eukaryotic universal probe (univ-SSU labelled with FITC). DAPI channel (first column), FITC-channel (second column), Cy-3 channel (third column). Fusion picture DAPI and FITC channels (last column).

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