Figure 5
MoDC pro-inflammatory cytokines in response to successive challenge of influenza virus and pneumococcus. (A-H) MoDCs were concurrently or successively challenged by both influenza virus and heat killed pneumococcus. (A-C) MoDCs were infected with H1N1 at MOI 0.1 for 0 hr, 6 hr or 24 hr, and then treated with 5 × 106 CFU heat killed pneumococcus for another 24 hr to make up a total incubation time at harvest of 24 hr, 30 hr or 48 hr respectively. (D-F) MoDCs were infected with H1N1 at MOI 0.1 for 6 hr, and then treated with 1 × 106, 5 × 106 or 10 × 106 CFU heat killed pneumococcus for another 24 hr, with a total incubation time at harvest was 30 hr (G-H) MoDCs were first infected with H1N1 at MOI 0.1 for 24 hr, and then treated with 1 × 106, 5 × 106 or 10 × 106 CFU heat killed pneumococcus for another 24 hr, with a total incubation time at harvest was 48 hr. Data (n = 5-7) represented mean ± SD for six individual experiments. Please note that some groups of data were presented twice in different panels for easy interpretation. *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA), post test for trend between white columns #p < 0.05 and black columns ^^ p < 0.01.