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Table 2 Multiplex primer

From: Sequential multiplex PCR assay for determining capsular serotypes of colonizing S. pneumoniae

Name Sequences (5' → 3') Amplicon size
1-f CTC TAT AGA ATG GAG TAT ATA AAC TAT GGT TA 280
1-r CCA AAG AAA ATA CTA ACA TTA TCA CAA TAT TGG C  
3-f ATG GTG TGA TTT CTC CTA GAT TGG AAA GTA G 371
3-r CTT CTC CAA TTG CTT ACC AAG TGC AAT AAC G  
4-f CTG TTA CTT GTT CTG GAC TCT CGA TAA TTG G 430
4-r GCC CAC TCC TGT TAA AAT CCT ACC CGC ATT G  
6-f AAT TTG TAT TTT ATT CAT GCC TAT ATC TGG 250
6-f TTA GCG GAG ATA ATT TAA AAT GAT GAC TA  
6C-f CAT TTT AGT GAA GTT GGC GGT GGA GTT 727
6C-r AGC TTC GAA GCC CAT ACT CTT CAA TTA  
7C/B (40)-f CTA TCT CAG TCA TCT ATT GTT AAA GTT TAC GAC GGG A 260
7C/B (40)-r GAA CAT AGA TGT TGA GAC ATC TTT TGT AAT TTC  
7A/F-f CCT ACG GGA GGA TAT AAA ATT ATT TTT GAG 826
7A/F-r CAA ATA CAC CAC TAT AGG CTG TTG AGA CTA AC  
9A/V-f CTT CGT TAG TTA AAA TTC TAA ATT TTT CTA AG 753
9A/V-r GTC CCA ATA CCA GTC CTT GCA ACA CAA G  
10A-f GGT GTA GAT TTA CCA TTA GTG TCG GCA GAC 628
10A-r GAA TTT CTT CTT TAA GAT TCG GAT ATT TCT C  
11A/D/F-f GGA CAT GTT CAG GTG ATT TCC CAA TAT AGT G 463
11A/D/F-r GAT TAT GAG TGT AAT TTA TTC CAA CTT CTC CC  
12F (12A/B, 44, 46)-f GCA ACA AAC GGC GTG AAA GTA GTT G 376
12F (12A/B, 44, 46)-r CAA GAT GAA TAT CAC TAC CAA TAA CAA AAC  
14-f* GAA ATG TTA CTT GGC GCA GGT GTC AGA ATT 189
14-r* GCC AAT ACT TCT TAG TCT CTC AGA TGA AT  
15A/F-f ATT AGT ACA GCT GCT GGA ATA TCT CTT C 434
15A/F-r GAT CTA GTG AAC GTA CTA TTC CAA AC  
15B/C-f TTG GAA TTT TTT AAT TAG TGG CTT ACC TA 496
15B/C-r CAT CCG CTT ATT AAT TGA AGT AAT CTG AAC C  
16F-f CTG TTC AGA TAG GCC ATT TAC AGC TTT AAA TC 988
16F-r CAT TCC TTT TGT ATA TAG TGC TAG TTC ATC C  
17F-f TTC GTG ATG ATA ATT CCA ATG ATC AAA CAA GAG 693
17F-r GAT GTA ACA AAT TTG TAG CGA CTA AGG TCT GC  
18-f CTT AAT AGC TCT CAT TAT TCT TTT TTT AAG CC 573
18-r TTA TCT GTA AAC CAT ATC AGC ATC TGA AAC  
19A-f GTT AGT CCT GTT TTA GAT TTA TTT GGT GTT GT 478
19A-r GAG CAG TCA ATA AGA TGA GAC GAT TGT TAG  
19F-f GTT AAG ATT GCT GAT CGA TTA ATT GAT ATC C 304
19F-r GTA ATA TGT CTT TAG GGC GTT TAT GGC GAT AG  
22-f GAG TAT AGC CAG ATT ATG GCA GTT TTA TTG TC 643
22-r CTC CAG CAC TTG CGC TGG AAA CAA CAG ACA AC  
23A-f*** TAT TCT AGC AAG TGA CGA AGA TGC G 722
23A-r*** CCA ACA TGC TTA AAA ACG CTG CTT TAC  
23B-f** TTG TTA GTG GTA TTA AAT TGG GGA CTA CTA GG 216
23B-r** ATA CCT ATC TGA AGT GTT ATT AAC CCA CCA AC  
23F-f GTA ACA GTT GCT GTA GAG GGA ATT GGC TTT TC 384
23F-r CAC AAC ACC TAA CAC TCG ATG GCT ATA TGA TTC  
24A/F-f** TCT CAA CCA AGA TAC AGA TTT TGA TTT TAC TC 686
24A/F-r** TAT AAA CCT TTA GTA AAC ACT CTG CTT GAT CG  
31-f GGA AGT TTT CAA GGA TAT GAT AGT GGT GGT GC 701
31-r CCG AAT AAT ATA TTC AAT ATA TTC CTA CTC  
33F (33A/37)-f GAA GGC AAT CAA TGT GAT TGT GTC GCG 338
33F (33A/37)-r CTT CAA AAT GAA GAT TAT AGT ACC CTT CTA C  
34-f GCT TTT GTA AGA GGA GAT TAT TTT CAC CCA AC 408
34-r CAA TCC GAC TAA GTC TTC AGT AAA AAA CTT TAC  
35A (35C/42)-f*** ATT ACG ACT CCT TAT GTG ACG CGC ATA 280
35A (35C/42)-r*** CCA ATC CCA AGA TAT ATG CAA CTA GGT T  
35B-f GAT AAG TCT GTT GTG GAG ACT TAA AAA GAA TG 677
35B-r CTT TCC AGA TAA TTA CAG GTA TTC CTG AAG CAA G  
35F (47F)-f GAA CAT AGT CGC TAT TGT ATT TTA TTT AAA GCA A 517
35F (47F)-r GAC TAG GAG CAT TAT TCC TAG AGC GAG TAA ACC  
38 (25A/F)-f CGT TCT TTT ATC TCA CTG TAT AGT ATC TTT ATG 574
38 (25A/F)-r ATG TTT GAA TTA AAG CTA ACG TAA CAA TCC  
cpsA-f GCA GTA CAG CAG TTT GTT GGA CTG ACC 160
cpsA-r GAA TAT TTT CAT TAT CAG TCC CAG TC  
  1. The majority of primer sequences are available on the CDC website (http://www.cdc.gov/ncidod/biotech/files/pcr-oligonucleotide-primers-March2010.pdf); *, ** and *** indicate primer sequences published by [25, 26], CDC website respectively. The name of each primer has been modified accordingly to the results obtained with the primer blast software.