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Table 2 Summary of PCR-RFA protocols used in this study1

From: Distinct genotypic profiles of the two major clades of Mycobacterium africanum

Locus Restriction enzyme MTC species Predicted digest pattern (bp)
katG 463 BstNI PGG1 MTC 12, 59, 106, 174
   PGG2, PGG3 M. tuberculosis 12, 59, 280
gyrA 95 AleI PGG1, PGG2 MTC 161, 193
   PGG3 M. tuberculosis 354
narGHJI -251 Sau3AI "modern" M. tuberculosis 155
   Remaining MTC 69, 86
gyrB 1450 Taq "M. canettii", M. tuberculosis 6, 21, 74, 96, 129, 270, 444
   Remaining MTC 6, 21, 74, 96, 129, 163, 107, 444
katG 203 BstNI "M. canettii", M. tuberculosis, M. africanum WA-1 140, 230
   Remaining MTC (PGG1a) 370
3'cfp32 311 BstNI M. tuberculosis, M. africanum WA-1 28, 34, 75, 2352
   Remaining MTC (PGG1a) 34, 75, 263
aroA 117 BssHII M. africanum WA-1 177, 254
   Remaining MTC 104, 150, 177
TbD1197 TseI M. africanum WA-1 46, 454
   Remaining MTC 46, 193, 2613
Rv1510 1129 NruI M. africanum WA-2, dassie bacillus 192, 841
   Remaining MTC 10334
hsp65 540 AflIII M. africanum WA-2 49, 392
   Remaining MTC 441
nat 751 BcgI M. africanum WA-2 111, 177, 208, 270
   Remaining MTC 111, 208, 447
rpoB 1049 Sau3AI M. africanum WA-2 11, 12, 18, 69, 87, 163
   Remaining MTC, M. africanum WA-2 11, 12, 18, 69, 250
rpoB 1163 BstUI M. africanum clade 2 28, 332
   Remaining MTC, M. africanum WA-2 28, 79, 253
mmpL6 551 Hpy166III "M. canettii", M. tuberculosis, M. africanum WA-1, M. africanum WA-2, dassie bacillus 155, 2983
   Remaining MTC 453
  1. WA, West African
  2. 1 This table contains the correction of errors from ref. [7]
  3. 2 "M. canettii" fails to PCR amplify
  4. 3 'modern' M. tuberculosis fails to PCR amplify
  5. 4 M. bovis fails to PCR amplify